COMPARISON OF BAY LEAF (Eugenia polyantha)  EXTRACT WITH THE DENTURE CLEANING SOLUTION TOWARD Candida albicans GROWTH ON PLATE OF ACRYLIC RESIN

Dilla Novia Amrilani¹, Sri Wahyuni R², Trisnawaty K³

Background: Accumulation of plaque and debris on denture surface facing mucosa can be contaminated by C. albicans that can cause denture stomatitis. One method to clean the denture is by using a denture cleaning solution.  One of the herbal plants that can be developed as a denture cleanser material is bay leaves, because it has been known having antifungal activity. Purpose: To determine the effectiveness of bay leaf extract compared with chemicals denture cleaning solution (Polident^®) in reducing the growth of C. albicans in denture base acrylic. Methods: This study was an experimental laboratory in vitro. Acrylic resin plate with a size of 10x10x1 mm that contaminated by C. albicans were grouped into 5 groups each consisted of 5 samples to be immersed for 30 minutes in ethanolic extract of bay leaf consentration of 25%, 50%, 75%, 100%, and Polident^®. C. albicans found in the sample was transferred to the saline solution and then cultured on agar medium. C. albicans colonies that grew were counted in units of CFU/ml. The data were analyzed by using the Kruskal-Wallis test (α=0,05) and was followed by Mann-Whitney test (p<0,05). Results: Bay leaf extract concentration of 75% had a significant difference in reducing the growth of C. albicans compared with concentration of 25% and 50%. Bay leaf extract concentration of 100% showed a less number of C. albicans colonies significantly different compared with the other concentrations groups, but the means difference of those number was not significant when it was compared to Polident^®.. Conclusion: Bay leaf extract 100% had similar ability with the Polident^® to inhibit the growth of  C. albicans.

Key word: Candida albicans, Bay leaves, Polident^®